The Division of Pulmonary Medicine in the Department of Pediatrics is seeking a full-time Research Assistant to join a productive laboratory to assist with research on the cell biology of pulmonary fibrosis and genetic lung diseases in adults and children.
The position will require performance of a combination of molecular and cell biology as well as designated work in maintaining and performing experiments using transgenic mouse colonies.
Under moderate supervision the Research Assistant II will:
Understand and perform routine procedures including mouse colony management and standard PCR.
Perform real time PCR, western blotting, ELISA, immunostaining, and cell culture.
Become proficient with tissue dissection/animal injections.
Become proficient in proper processing and catologing of human samples for genetic, genomic, and proteomic research
Assist in other laboratory and research functions.
Key Functions and Expected Performances:
1. Under moderate supervision:
Understand and follow experimental protocols, including animal handling, cell isolation and tissue harvest, cell culture, and molecular assays
Set up, conduct experiments, and continue research projects following protocols
Prepare and operate equipment in experimental procedures and maintain laboratory inventory and equipment
Follow University safety protocols, and laboratory protocols including maintaining sterile conditions as required
Maintain inventories and process and catalog specimens
Perform accurate calculations in the preparation of reagents and solutions
Keep detailed documentation of all procedures and protocols in a lab notebook and electronically as appropriate
Compile and communicate results to PI and lab manager, including the appropriate recording of data and reporting of standard or variant results
Recognize and communicate potential problems and suggest solutions
Bachelor's degree plus minimum 24 months experience; Masters Degree preferred
Basic laboratory skills and prior molecular biology laboratory experience is required.
A degree in Biochemistry, Cell Biology or related science field is preferred.
Previous research experience working with mice is preferred.
Required skills: strong analytical and communication skills; the ability to work independently; computer skills including word processing, data entry, statistical analysis, and graphic presentation.
Vanderbilt University Medical Center is home to Vanderbilt University Hospital, The Monroe Carell Jr. Children’s Hospital at Vanderbilt, the Vanderbilt Psychiatric Hospital and the Vanderbilt Stallworth Rehabilitation Hospital. These hospitals experienced more than 61,000 inpatient admissions during fiscal year 2015. Vanderbilt’s adult and pediatric clinics treated nearly 2 million patients during this same period.Vanderbilt University Hospital and the Monroe Carell Jr. Children’s Hospital at Vanderbilt are recognized again this year by U.S. News & World Report’s Best Hospitals as among the nation’s best with 18 nationally ranked specialties. Vanderbilt University Medical Center is world renowned because of the innovation, work ethic and collegiality of its employees. From our health care advances to our compassionate care, Vanderbilt owes its accomplishments and reputation to staff and faculty who bring skill and drive and innovation to the medical center day after day. World-leading academic departments and comprehensive centers of excellence pursue scientific discoveries and transformational educational and clinical advances across the entire spectrum of health and disease.As th...e largest employer in middle Tennessee, we welcome those who are interested in ongoing development in a caring, culturally sensitive and professional atmosphere. Most of us spend so much of our lives at work, we want to be part of maintaining a workplace in which people support one another and encourage reaching for excellence. Many high-achieving employees stay at Vanderbilt because of the professional growth they experience and because of their appreciation of Vanderbilt’s benefits, public events and discussions, athletic opportunities, beautiful setting and, above all, sense of community and purpose.Vanderbilt and its employees share a set of mutual expectations that have been created with productivity, legality, fairness and safety always in mind. We believe that our investment in training and compensating employees multiplies in value when we enable individuals to deliver their best performance for the benefit of us all.